Journal: Biomedicines

Article Title: Characterization of Mesothelin Glycosylation in Pancreatic Cancer: Decreased Core Fucosylated Glycoforms in Pancreatic Cancer Patients’ Sera

doi: 10.3390/biomedicines10081942

Figure Lengend Snippet: Lectins used in WB.

Article Snippet: Fluorescein labelled Phaseolus vulgaris Erythroagglutinin (PHA-E) , Galβ4GlcNAcβ2Manα6 (GlcNAcβ4) (GlcNAcβ4Manα3) Manβ4 , 2 µg/mL , FL-1121 (Vector Laboratories).

Techniques: Concentration Assay, Plasmid Preparation

Journal: Stem cells (Dayton, Ohio)

Article Title: Mesenchymal stem cells and endothelial progenitor cells decrease renal injury in experimental swine renal artery stenosis through different mechanisms

doi: 10.1002/stem.1263

Figure Lengend Snippet: Top: Representative images of CM-DiI labeled (red) endothelial progenitor cells (EPC) or mesenchymal stem cells (MSC) in the post-stenotic kidneys of pigs with renal artery stenosis (RAS) 4 weeks after cell delivery. Green shows peanut agglutinin (PA, green arrow), a distal tubular marker, and cyan shows a proximal tubular marker phaseolus vulgaris erythroagglutinin (PHA-E, cyan arrow). EPC showed mainly tubular engraftment (yellow arrow), while MSC tend to integrate into both proximal tubules (yellow arrow) and interstitial area (red arrow). Bottom: Both EPC and MSC improved RBF and GFR in pigs with RAS, yet MSC more effectively restored GFR. *p<0.05 vs. Normal, †p<0.05 vs. RAS. Scale bar=200µm

Article Snippet: Furthermore, frozen kidney sections from pigs infused with cells were stained with the distal tubular marker peanut agglutinin (5ug/ml, Vector) and proximal tubular marker phaseolus vulgaris erythroagglutinin (PHA-E, 5ug/ml, Vector).

Techniques: Labeling, Marker

Journal: Glycoconjugate Journal

Article Title: The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells. GnT-III modifies highly branched N-glycans

doi: 10.1007/s10719-018-9814-y

Figure Lengend Snippet: List of plant lectins used in lectin blot assay

Article Snippet: Membranes were incubated (1 h, RT) with following biotinylated lectins (Vector Labs, Burlingame, CA): Phaseolus vulgaris erythroagglutinin (PHA-E), Phaseolus vulgaris leucoagglutinin (PHA-L), Sambucus nigra agglutinin (SNA), Maackia amurensis agglutinin (MAA), Galanthus nivalis agglutinin (GNA) and Aleuria aurantia agglutinin (AAA) (see Table ).

Techniques: Binding Assay

Journal: Glycoconjugate Journal

Article Title: The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells. GnT-III modifies highly branched N-glycans

doi: 10.1007/s10719-018-9814-y

Figure Lengend Snippet: Membrane proteins ( a ) and secreted proteins ( b ) isolated of control cells (WM266–4-pIRESneo) and GnT-III overepressing cells (WM266–4-GnT-III) were resolved by SDS-PAGE and blotted glycoproteins were probed with specific lectins. Optical density of each lane was measured and presented in relation to optical density of loading control (CBB stained blots). Three independent experiments were performed. Data are presented as means +/− standard deviation (SD). Asterisk indicates statistically significant difference (* p < 0.05, ** p < 0.01; Student t-test)

Article Snippet: Membranes were incubated (1 h, RT) with following biotinylated lectins (Vector Labs, Burlingame, CA): Phaseolus vulgaris erythroagglutinin (PHA-E), Phaseolus vulgaris leucoagglutinin (PHA-L), Sambucus nigra agglutinin (SNA), Maackia amurensis agglutinin (MAA), Galanthus nivalis agglutinin (GNA) and Aleuria aurantia agglutinin (AAA) (see Table ).

Techniques: Isolation, SDS Page, Staining, Standard Deviation

Journal: The Febs Journal

Article Title: Glycan biomarkers for Alzheimer disease correlate with T‐tau and P‐tau in cerebrospinal fluid in subjective cognitive impairment

doi: 10.1111/febs.15197

Figure Lengend Snippet: Schematics of ELLA. Diluted CSF samples were incubated in 96‐well high‐absorbing plates. The samples were washed, and biotinylated lectin (PHA‐E) was added. After washing, avidin‐conjugated HRP was added followed by the addition of Amplex red. The fluorescence from the resulting product was measured in a fluorescence reader.

Article Snippet: Biotinylated PHA‐E lectin (Vector Laboratories, Burlingame, CA, USA) was used for detecting N‐glycans containing bisecting N‐acetylglucosamine (GlcNAc).

Techniques: Incubation, Avidin-Biotin Assay, Fluorescence

Journal:

Article Title: Identification of cell surface glycoprotein markers for glioblastoma-derived stem-like cells using a lectin microarray and LC-MS/MS approach

doi: 10.1021/pr100012p

Figure Lengend Snippet: Carbohydrate specificities of the 16 lectins used for lectin microarray.

Article Snippet: Phaseolus vulgaris leucoagglutinin , PHA-L , Galβ (1–4)GlcNAcβ (1–6)Man , EY Laboratories Inc..

Techniques: Microarray, Plasmid Preparation